Photoreactions for total antioxidant status measurement in humans

David Olivier; Samuel Douillard; Isabelle Lhommeau; Edith Bigot; Thierry Patrice

doi:10.1117/12.823472

13 July 2009 Photoreactions for total antioxidant status measurement in humans

David Olivier, Samuel Douillard, Isabelle Lhommeau, Edith Bigot, Thierry Patrice

Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 73800O (2009) https://doi.org/10.1117/12.823472
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States

Abstract

Type 2 photoreactions are singlet oxygen (¹O₂) mediated. This singlet oxygen will de activate by reacting with a number of targets and produce ROS and peroxides that will in turn give Reactive Oxygen Species (ROS). In order to verify whether sera had the same capability to do so we provoked a photoreaction using Rose Bengal (RB) added to sera of 53 healthy donors and at the end of light delivery 2' -7' dichlorofluorescin (DCFH) had been added. In order to avoid any optical artefact we analysed the influence of haemolysis on the fluorescent 2' -7' dichlorofluorescin (DCF) induced by oxidising species. Reactivity to singlet oxygen was slightly different between men and women, varied slightly with age and platelet number. It was remarkably stable over a large period of time and thus represents a characteristic of each serum. Studies are in progress to verify whether ROS dependent diseases would impair this reactivity to singlet oxygen.

Citation Download Citation

David Olivier, Samuel Douillard, Isabelle Lhommeau, Edith Bigot, and Thierry Patrice "Photoreactions for total antioxidant status measurement in humans", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73800O (13 July 2009); https://doi.org/10.1117/12.823472

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