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This study aimed to investigate the feasibility of non-invasive evaluating vessel blood oxygenation in the human eye
using a dual-wavelength confocal scanning laser ophthalmoscope (cSLO). A cSLO prototype was adapted to use a new
combination of two red and infrared wavelengths simultaneously performing a spectral analysis of the status of retinal
blood vessel to give the ability to image perfusion in the human eye. This technique was evaluated using measurements
made on normal volunteers' eyes. The reproducibility of the measurements of reflected light from the eye was
investigated. Spectral measurements of light reflected from retinal vessels were determined and liner relationship of
oxygen saturation of blood and spectral transmittance based on Beer-Lambert law was defined. The relative oxygen
levels of retinal blood vessels (artery and vein) were successfully determined using these two wavelengths. The
technique showed promise in the determination of the relative oxygen saturation levels of the retina in amongst eye
subjects.
Seyed Hossein Rasta,A. Manivannan, andPeter F. Sharp
"Spectroscopic imaging of the retinal vessels using a new dual-wavelength", Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 736805 (7 July 2009); https://doi.org/10.1117/12.831635
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Seyed Hossein Rasta, A. Manivannan, Peter F. Sharp, "Spectroscopic imaging of the retinal vessels using a new dual-wavelength," Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 736805 (7 July 2009); https://doi.org/10.1117/12.831635