Applying image restoration to fluorescence lifetime imaging microscopy (FLIM)

Ching-Wei Chang; Mary-Ann Mycek

doi:10.1117/12.831498

1 July 2009 Applying image restoration to fluorescence lifetime imaging microscopy (FLIM)

Ching-Wei Chang, Mary-Ann Mycek

Proceedings Volume 7367, Advanced Microscopy Techniques; 73671E (2009) https://doi.org/10.1117/12.831498
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

We describe a novel approach using 2D-intensity-deconvolution to improve spatial resolution in wide-field FLIM. The method maintains lifetime accuracy and can restore features within experimentally reasonable intensity ranges.

Citation Download Citation

Ching-Wei Chang and Mary-Ann Mycek "Applying image restoration to fluorescence lifetime imaging microscopy (FLIM)", Proc. SPIE 7367, Advanced Microscopy Techniques, 73671E (1 July 2009); https://doi.org/10.1117/12.831498

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available