Measuring dynamics of Caspase-9 activity in living cells using FRET technique during apoptosis induced by high fluence low-power laser irradiation

Shengnan Wu; Lei Huang; Xuegang Sun; Jiru Chu

doi:10.1117/12.822153

6 March 2009 Measuring dynamics of Caspase-9 activity in living cells using FRET technique during apoptosis induced by high fluence low-power laser irradiation

Shengnan Wu, Lei Huang, Xuegang Sun, Jiru Chu

Author Affiliations +

Proceedings Volume 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine; 728023 (2009) https://doi.org/10.1117/12.822153
Event: Photonics and Optoelectronics Meetings, 2008, Wuhan, China

Abstract

We investigated the activity of caspase-9 for its role in the regulation of apoptosis induced by high fluence Low-power laser irradiation (HF-LPLI). Using a fluorescence resonance energy transfer (FRET) reporter STAT9, caspase-9 activity was monitored in a noninvasive technique in living human lung adenocarcinoma cells (ASTC-a-1). Under physiological conditions, proteolytic activity of caspase-9 kept invalid in order to prevent the cell undergoing apoptosis. However, HF-LPLI caused a significant decrease of Venus/ECFP ratio, indicating caspase-9 was activated which sustained from 70 minutes to 200 minutes post irradiation. This behavior was familiar with that under staurosporine (STS) treatment, which was used here as a positive control to show a characteristical activation of caspase-9. These results demonstrate that the control of caspase-9 activity is an important mechanism for the regulation of apoptosis triggered by HF-LPLI.

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Shengnan Wu, Lei Huang, Xuegang Sun, and Jiru Chu "Measuring dynamics of Caspase-9 activity in living cells using FRET technique during apoptosis induced by high fluence low-power laser irradiation", Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 728023 (6 March 2009); https://doi.org/10.1117/12.822153

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KEYWORDS

Cell death

Fluorescence resonance energy transfer

Venus

Laser irradiation

Luminescence

Molecules

Oxygen

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