Relative concentration of fluorophores in a scattering medium using fluorescence lifetime contrast

David J. Hall; Sung-Ho Han

doi:10.1117/12.808609

20 February 2009 Relative concentration of fluorophores in a scattering medium using fluorescence lifetime contrast

David J. Hall, Sung-Ho Han

Proceedings Volume 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications; 71900S (2009) https://doi.org/10.1117/12.808609
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

In vivo optical molecular imaging of fluorescent probes predominantly employs continuous wave techniques to measure fluorescence intensity. Alternatively, time domain techniques permit measurement of fluorescence lifetime in addition to fluorescence intensity. Fluorescence lifetime allows discrimination of fluorescent probes with contrasting lifetime or inference of a probe's environment due to lifetime sensitivity. Here, we present the use of fluorescence lifetime contrast to evaluate the relative concentrations of a mixture of fluorophores in a scattering medium. This approach offers the potential to perform dual-probe in vivo optical molecular imaging at a single wavelength employing lifetime contrast rather than via spectral intensity contrast.

Citation Download Citation

David J. Hall and Sung-Ho Han "Relative concentration of fluorophores in a scattering medium using fluorescence lifetime contrast", Proc. SPIE 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications, 71900S (20 February 2009); https://doi.org/10.1117/12.808609

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KEYWORDS

Luminescence

Scattering

In vivo imaging

Molecular imaging

Environmental sensing

Manufacturing

Optics manufacturing

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