Fluorescent nanodiamonds for FRET-based monitoring of a single biological nanomotor FoF1-ATP synthase

M. Börsch; R. Reuter; G. Balasubramanian; R. Erdmann; F. Jelezko; J. Wrachtrup

doi:10.1117/12.812720

13 February 2009 Fluorescent nanodiamonds for FRET-based monitoring of a single biological nanomotor F_oF₁-ATP synthase

M. Börsch, R. Reuter, G. Balasubramanian, R. Erdmann, F. Jelezko, J. Wrachtrup

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 71832N (2009) https://doi.org/10.1117/12.812720
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Color centers in diamond nanocrystals are a new class of fluorescence markers that attract significant interest due to matchless brightness, photostability and biochemical inertness. Fluorescing diamond nanocrystals containing defects can be used as markers replacing conventional organic dye molecules, quantum dots or autofluorescent proteins. They can be applied for tracking and ultrahigh-resolution localization of the single markers. In addition the spin properties of diamond defects can be utilized for novel magneto-optical imaging (MOI) with nanometer resolution. We develop this technique to unravel the details of the rotary motions and the elastic energy storage mechanism of a single biological nanomotor F_oF₁-ATP synthase. F_oF₁-ATP synthase is the enzyme that provides the 'chemical energy currency' adenosine triphosphate, ATP, for living cells. The formation of ATP is accomplished by a stepwise internal rotation of subunits within the enzyme. Previously subunit rotation has been monitored by single-molecule fluorescence resonance energy transfer (FRET) and was limited by the photostability of the fluorophores. Fluorescent nanodiamonds advance these FRET measurements to long time scales.

Citation Download Citation

M. Börsch, R. Reuter, G. Balasubramanian, R. Erdmann, F. Jelezko, and J. Wrachtrup "Fluorescent nanodiamonds for FRET-based monitoring of a single biological nanomotor F_oF₁-ATP synthase", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71832N (13 February 2009); https://doi.org/10.1117/12.812720

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available