Adaptive optics for multiphoton microscopy

Delphine Débarre; Tony Wilson; Martin J. Booth

doi:10.1117/12.810367

13 February 2009 Adaptive optics for multiphoton microscopy

Delphine Débarre, Tony Wilson, Martin J. Booth

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 71831J (2009) https://doi.org/10.1117/12.810367
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Specimen-induced aberrations are frequently encountered in high resolution microscopy, particularly when high numerical aperture lenses are used to image deep into biological specimens. These aberrations distort the focal spot causing a reduction in resolution and, often more importantly, reduced signal level and contrast. This is particularly problematic in multiphoton microscopy, where the non-linear nature of the signal generation process means that the signal level is strongly affected by changes in the focal spot intensity. We have developed an adaptive two-photon fluorescence microscope to correct for these aberrations. Unlike a conventional adaptive optics system, the microscope does not include a wavefront sensor but uses an efficient sensorless optimisation scheme to obtain optimum aberration correction.

Citation Download Citation

Delphine Débarre, Tony Wilson, and Martin J. Booth "Adaptive optics for multiphoton microscopy", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71831J (13 February 2009); https://doi.org/10.1117/12.810367

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