25 February 2009Reconstruction of thin fluorophore-filled capillaries in thick scattering medium using fluorescence diffuse optical tomography within the diffusion approximation
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Current efforts in tissue engineering target the growth of 3D volumes of tissue cultures in bioreactor conditions.
Fluorescence optical tomography has the potential to monitor cells viability and tissue growth non-destructively
directly within the bioreactor via bio-molecular fluorescent labelling strategies. We currently work on developing
the imaging instrumentation for tissue cultures in bioreactor conditions. Previously, we localized in 3D
thin fluorescent-labelled capillaries in a cylindrically shaped bioreactor phantom containing a diffusive medium
with our time-of-flight localization technique. Here, we present our first reconstruction results of the spatial
distribution of fluorophore concentrations for labelled capillaries embedded in a bioreactor phantom.
Johanne Desrochers,Patrick Vermette,Réjean Fontaine, andYves Bérubé-Lauzière
"Reconstruction of thin fluorophore-filled capillaries in thick scattering medium using fluorescence diffuse optical tomography within the diffusion approximation", Proc. SPIE 7179, Optics in Tissue Engineering and Regenerative Medicine III, 71790I (25 February 2009); https://doi.org/10.1117/12.809870
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Johanne Desrochers, Patrick Vermette, Réjean Fontaine, Yves Bérubé-Lauzière, "Reconstruction of thin fluorophore-filled capillaries in thick scattering medium using fluorescence diffuse optical tomography within the diffusion approximation," Proc. SPIE 7179, Optics in Tissue Engineering and Regenerative Medicine III, 71790I (25 February 2009); https://doi.org/10.1117/12.809870