Laser-scanning optical-resolution photoacoustic microscopy

Hao F. Zhang; Zhixing Xie; Shuliang Jiao; Carmen A Puliafito

doi:10.1117/12.815396

24 February 2009 Laser-scanning optical-resolution photoacoustic microscopy

Hao F. Zhang, Zhixing Xie, Shuliang Jiao, Carmen A Puliafito

Proceedings Volume 7177, Photons Plus Ultrasound: Imaging and Sensing 2009; 71770L (2009) https://doi.org/10.1117/12.815396
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We have developed a laser-scanning optical-resolution photoacoustic microscopy that can potentially be easily integrated with several existing optical microscopic modalities. During data acquisition, the ultrasonic transducer is kept stationary and only the laser light is raster-scanned by an x-y galvanometer scanner. In this configuration, the field-ofview is limited by the beam diameter of the ultrasonic detector, which is related to the active element size, numerical aperture, and the center frequency of the ultrasonic transducer. The spatial resolution is determined by the size of the optical focus. A lateral resolution of 7.8 μm and a circular field-of-view with a diameter of 6 mm were achieved in an optically clear medium. Using a laser system working at a pulse repetition rate of 1024 Hz, the data acquisition time for an image consisting of 256×256 pixels was less than two minutes. In vivo imaging of microvasculature in mouse ears were also achieved.

Citation Download Citation

Hao F. Zhang, Zhixing Xie, Shuliang Jiao, and Carmen A Puliafito "Laser-scanning optical-resolution photoacoustic microscopy", Proc. SPIE 7177, Photons Plus Ultrasound: Imaging and Sensing 2009, 71770L (24 February 2009); https://doi.org/10.1117/12.815396

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