Automated Raman spectral preprocessing of bone and other musculoskeletal tissues

Francis W. L. Esmonde-White; Matthew V. Schulmerich; Karen A. Esmonde-White; Michael D. Morris

doi:10.1117/12.809436

18 February 2009 Automated Raman spectral preprocessing of bone and other musculoskeletal tissues

Francis W. L. Esmonde-White, Matthew V. Schulmerich, Karen A. Esmonde-White, Michael D. Morris

Proceedings Volume 7166, Optics in Bone Biology and Diagnostics; 716605 (2009) https://doi.org/10.1117/12.809436
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Raman spectroscopy of bone is complicated by fluorescence background and spectral contributions from other tissues. Full utilization of Raman spectroscopy in bone studies requires rapid and accurate calibration and preprocessing methods. We have taken a step-wise approach to optimize and automate calibrations, preprocessing and background correction. Improvements to manual spike removal, white light correction, software image rotation and slit image curvature correction are described. Our approach is concisely described with a minimum of mathematical detail.

Citation Download Citation

Francis W. L. Esmonde-White, Matthew V. Schulmerich, Karen A. Esmonde-White, and Michael D. Morris "Automated Raman spectral preprocessing of bone and other musculoskeletal tissues", Proc. SPIE 7166, Optics in Bone Biology and Diagnostics, 716605 (18 February 2009); https://doi.org/10.1117/12.809436

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