System for fluorescence quantification of thin tissue layers guided by high frequency ultrasound

Josiah D. Gruber; Venkataramanan Krishnaswamy; Edward V. Maytin M.D.; Tayyaba Hasan; Brian W. Pogue

doi:10.1117/12.809011

18 February 2009 System for fluorescence quantification of thin tissue layers guided by high frequency ultrasound

Josiah D. Gruber, Venkataramanan Krishnaswamy, Edward V. Maytin M.D., Tayyaba Hasan, Brian W. Pogue

Proceedings Volume 7164, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII; 71640N (2009) https://doi.org/10.1117/12.809011
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

A system is presented which has been developed for dermatological applications with the need to quantify levels of protoporphyrin IX in diagnosis or therapy. The design was to couple fluorescence sampling onto a high frequency ultrasound system and take multiple optical source-detector samples of the tissue of the fluorescence and transmission signals. The intensity values can then be used to estimate and image the PpIX levels present in tissue samples. The system design, calibration, and initial testing in tissue phantoms are demonstrated here. The component design has been modular and allows easy implementation as a kit which can be assembled from basic components. The control software is more elaborate and provides a seamless way to go from system start up through to fluorescence quantification of PpIX concentrations.

Citation Download Citation

Josiah D. Gruber, Venkataramanan Krishnaswamy, Edward V. Maytin M.D., Tayyaba Hasan, and Brian W. Pogue "System for fluorescence quantification of thin tissue layers guided by high frequency ultrasound", Proc. SPIE 7164, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII, 71640N (18 February 2009); https://doi.org/10.1117/12.809011

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