In vivo confocal Raman spectroscopy for skin disease diagnosis and characterization: preliminary results from mouse tumor models

Hequn Wang; Naiyan Huang; Jianhua Zhao; Harvey Lui; Mladen Korbelik; Haishan Zeng

doi:10.1117/12.810953

19 February 2009 In vivo confocal Raman spectroscopy for skin disease diagnosis and characterization: preliminary results from mouse tumor models

Hequn Wang, Naiyan Huang, Jianhua Zhao, Harvey Lui, Mladen Korbelik, Haishan Zeng

Author Affiliations +

Proceedings Volume 7161, Photonic Therapeutics and Diagnostics V; 716108 (2009) https://doi.org/10.1117/12.810953
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

As applied to microscopy, the confocal technique is a powerful method that can non-invasively provide depth-resolved information on tissue because of its optical sectioning capability. Raman spectroscopy is a non-invasive optical technique that is very sensitive to the structure and conformation of biochemical constitutes. Combining these two distinct techniques can non-invasively provide depth-resolved biochemical information of the skin in vivo. In this paper, we present a novel confocal Raman spectrometer and preliminary results on in vivo Raman spectral measurements of mouse tumor model. A total of 255 Raman spectra were taken from 15 mice in vivo. We found that Raman spectra of different sub-layers in mouse skin differ significantly. Obvious spectral changes for the dermis were also observed between normal skin and skin immediately overlying subcutaneous tumor.

Citation Download Citation

Hequn Wang, Naiyan Huang, Jianhua Zhao, Harvey Lui, Mladen Korbelik, and Haishan Zeng "In vivo confocal Raman spectroscopy for skin disease diagnosis and characterization: preliminary results from mouse tumor models", Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 716108 (19 February 2009); https://doi.org/10.1117/12.810953

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