Automatable lipid bilayer formation for ion channel studies

Jason L. Poulos; Hyunwoo Bang; Tae-Joon Jeon; Jacob J. Schmidt

doi:10.1117/12.795375

29 August 2008 Automatable lipid bilayer formation for ion channel studies

Jason L. Poulos, Hyunwoo Bang, Tae-Joon Jeon, Jacob J. Schmidt

Proceedings Volume 7035, Biosensing; 703509 (2008) https://doi.org/10.1117/12.795375
Event: NanoScience + Engineering, 2008, San Diego, California, United States

Abstract

Transmembrane proteins and ion channels are important drug targets and have been explored as single molecule sensors. For these proteins to function normally they must be integrated within lipid bilayers; however, the labor and skill required to create artificial lipid bilayers have the limited the possible applications utilizing these proteins. In order to reduce the complexity and cost of lipid bilayer formation and measurement, we have modified a previously published lipid bilayer formation technique using mechanically contacted monolayers so that the process is automatable, requiring minimal operator input. Measurement electronics are integrated with the fluid handling system, greatly reducing the time and operator feedback characteristically required of traditional bilayer experiments. To demonstrate the biological functionality of the resultant bilayers and the system's capabilities as a membrane platform, the ion channel gramicidin A was incorporated and measured with this system.

Citation Download Citation

Jason L. Poulos, Hyunwoo Bang, Tae-Joon Jeon, and Jacob J. Schmidt "Automatable lipid bilayer formation for ion channel studies", Proc. SPIE 7035, Biosensing, 703509 (29 August 2008); https://doi.org/10.1117/12.795375

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