Application of light emitting diodes in digital holographic microscopy

Stephan Stürwald; Björn Kemper; Christian Remmersmann; Patrik Langehanenberg; Gert von Bally

doi:10.1117/12.781186

25 April 2008 Application of light emitting diodes in digital holographic microscopy

Stephan Stürwald, Björn Kemper, Christian Remmersmann, Patrik Langehanenberg, Gert von Bally

Proceedings Volume 6995, Optical Micro- and Nanometrology in Microsystems Technology II; 699507 (2008) https://doi.org/10.1117/12.781186
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

Digital holographic microscopy permits quantitative phase contrast imaging of reflective and (partially) transparent samples. The utilization of low coherent light sources opens up prospects for a reduced phase noise by avoiding multiple reflections in the experimental setup. Thus, light emitting diodes (LEDs) have been investigated for applicability as low cost light sources in digital holographic microscopy. The LEDs were characterized for the spectral properties and the resulting coherence length. Furthermore, dispersion effects and their influences on the interferogram formation have been analyzed. Since the interference fringe number of off-axis holograms is limited by the coherence length of the LEDs (few micrometers) in addition to spatial phase shifting digital holographic reconstruction techniques, temporal phase shifting procedures were applied. The characterization of the lateral and axial resolution has been performed for both temporal and spatial phase shifting techniques by investigations on technical specimen. Finally, the application on biological samples is demonstrated by investigations on pancreas tumor cells.

Citation Download Citation

Stephan Stürwald, Björn Kemper, Christian Remmersmann, Patrik Langehanenberg, and Gert von Bally "Application of light emitting diodes in digital holographic microscopy", Proc. SPIE 6995, Optical Micro- and Nanometrology in Microsystems Technology II, 699507 (25 April 2008); https://doi.org/10.1117/12.781186

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