The application of FRET biosensors to visualize Src activation

Yingxiao Wang; Shaoying Lu

doi:10.1117/12.764713

29 February 2008 The application of FRET biosensors to visualize Src activation

Yingxiao Wang, Shaoying Lu

Proceedings Volume 6868, Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes; 68680A (2008) https://doi.org/10.1117/12.764713
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Src kinase, the first tyrosine kinase discovered, has been shown to play critical roles in a variety of cellular processes, including cell motility/migration, mechanotranduction, and cancer development. Based on fluorescent resonance energy transfer (FRET), we have developed and characterized a genetically encoded single-molecule Src biosensor, which enables the imaging and quantification of temporal-spatial activation of Src in live cells. In this paper, we summarize the application of this biosensor to study a variety of cellular functions. First, we introduced a local mechanical stimulation by applying laser-tweezer-induced traction on fibronectin-coated beads adhered to the cells. Using a membrane-anchored Src biosensor, we observed a wave propagation of Src activation in a direction opposite to the applied force. This Src reporter was also applied to visualize the interplays between cell-cell and cell-ECM adhesions. The results indicate that integrin-ligation can induce Src activation around cell-cell junctions and cause the disruption of adherens junctions. Lastly, the flow-induced dynamic Src activation at subcellular levels was visualized by the FRET biosensor simultaneously with actin-fused mCherry, a red fluorescence protein. Our results indicate that shear stress induced a moderate up-regulation of Src activation in the whole cell, but a significant translocation of active Src from perinuclear regions toward cell periphery. In summary, our novel Src biosensor has made it possible to monitor key signaling transduction cascades involving Src in live cells with temporal-spatial characterization in mechanobiology.

Citation Download Citation

Yingxiao Wang and Shaoying Lu "The application of FRET biosensors to visualize Src activation", Proc. SPIE 6868, Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes, 68680A (29 February 2008); https://doi.org/10.1117/12.764713

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KEYWORDS

Biosensors

Fluorescence resonance energy transfer

Visualization

Luminescence

Proteins

Molecules

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