Wide-field time-correlated single photon counting imaging for luminescence microscopy

Nicolas Sergent; James A. Levitt; Mark A. Green; Klaus Suhling

doi:10.1117/12.763561

12 February 2008 Wide-field time-correlated single photon counting imaging for luminescence microscopy

Nicolas Sergent, James A. Levitt, Mark A. Green, Klaus Suhling

Proceedings Volume 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV; 68610K (2008) https://doi.org/10.1117/12.763561
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We demonstrateWide-Field Time-Correlated Single Photon Counting (WiFi TCSPC) imaging based on an image intensifier and a high-speed camera running at 30,000 frames per second. The timing of photon events is thus performed in parallel, simultaneously on every pixel. The system is applied to lanthanide lifetime measurements and time-resolved imaging of the lanthanide complex Europium Polyoxometalate (Eu POMs). We measure a lifetime of 2.98 ms for Eu POMs in solid state, which is in excellent agreement with the literature value.

Citation Download Citation

Nicolas Sergent, James A. Levitt, Mark A. Green, and Klaus Suhling "Wide-field time-correlated single photon counting imaging for luminescence microscopy", Proc. SPIE 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV, 68610K (12 February 2008); https://doi.org/10.1117/12.763561

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