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20 February 2008 Observing dynamics of transparent samples by harmonically matched grating-based full-field quadrature phase interferometer
Jigang Wu, Zahid Yaqoob, Xin Heng, Lap Man Lee, Xiquan Cui, Changhuei Yang
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Proceedings Volume 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV; 686102 (2008) https://doi.org/10.1117/12.759210
Event: SPIE BiOS, 2008, San Jose, California, United States
Abstract
Our group has reported the use of harmonically matched diffraction grating for full-field quantitative phase imaging. In this paper, we show the improvement of this technique and the application in observing dynamics of transparent samples. By using the grating as a beam splitter/combiner in an interferometer, we are able to obtain non-trivial phase difference between the output ports of the grating. We have built a Mach-Zehnder interferometer using the holographic grating with 600 and 1200 lines/mm spacing. Two CCD cameras at the output ports of the grating-based Mach-Zehnder interferometer are used to record the full-field quadrature interferograms, which are subsequently processed to reconstruct the phase image. Since the two quadrature interferograms are acquired at the same time, the imaging speed of the system is limited only by the frame rate of the CCD cameras. We have demonstrated the capability of our system by observing dynamics of transparent samples.
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Jigang Wu, Zahid Yaqoob, Xin Heng, Lap Man Lee, Xiquan Cui, and Changhuei Yang "Observing dynamics of transparent samples by harmonically matched grating-based full-field quadrature phase interferometer", Proc. SPIE 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV, 686102 (20 February 2008); https://doi.org/10.1117/12.759210
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KEYWORDS
Phase shifts
Diffraction gratings
Interferometers
Imaging systems
Beam splitters
Charge-coupled devices
Image processing
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