Nonlinear interferometric vibrational imaging of biological tissue

Zhi Jiang; Daniel L. Marks; Joseph B. Geddes III; Stephen A. Boppart

doi:10.1117/12.763306

15 February 2008 Nonlinear interferometric vibrational imaging of biological tissue

Zhi Jiang, Daniel L. Marks, Joseph B. Geddes III, Stephen A. Boppart

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 68600Y (2008) https://doi.org/10.1117/12.763306
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We demonstrate imaging with the technique of nonlinear interferometric vibrational imaging (NIVI). Experimental images using this instrumentation and method have been acquired from both phantom and biological tissues. In our system, coherent anti-Stokes Raman scattering (CARS) signals are detected by spectral interferometry, which is able to fully restore high resolution Raman spectrum on each focal spot of a sample covering multiple Raman bands using broadband pump and Stokes laser beams. Spectral-domain detection has been demonstrated and allows for a significant increase in image acquiring speed, in signal-to-noise, and in interferometric signal stability.

Citation Download Citation

Zhi Jiang, Daniel L. Marks, Joseph B. Geddes III, and Stephen A. Boppart "Nonlinear interferometric vibrational imaging of biological tissue", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68600Y (15 February 2008); https://doi.org/10.1117/12.763306

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