High throughput analysis of proteins using mid-infrared laser

S. Yoshihashi-Suzuki; T. Fujita; I. Sato; K. Awazu

doi:10.1117/12.764184

5 March 2008 High throughput analysis of proteins using mid-infrared laser

S. Yoshihashi-Suzuki, T. Fujita, I. Sato, K. Awazu

Proceedings Volume 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI; 68591Y (2008) https://doi.org/10.1117/12.764184
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Large-scale analysis of proteins, which are functional biomolecules, has assumed an important role in the life sciences. Mass spectrometry is one of the techniques used to identify ptoreins. Direct desorption and ionization of proteins from polyacrylamide gel is expected to be a the high-throughput technique in proteomics, which will eliminate several problems such as sample loss, adduct formation, and contamination. In this study, we performed direct ionization of a protein, bovine insulin, in a polyacrylamide gel without matrix addition with a tunable MIR nanosecond pulsed laser. Mass spectra of insulin in an acrylamide gel and an acrylamide solution were recorded in the wavelength range 5.8-5.9 μm and at a wavelength of 6.0 μm, respectively. This wavelength corresponds to the >C=O stretching vibration mode of acrylamide.

Citation Download Citation

S. Yoshihashi-Suzuki, T. Fujita, I. Sato, and K. Awazu "High throughput analysis of proteins using mid-infrared laser", Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 68591Y (5 March 2008); https://doi.org/10.1117/12.764184

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available