Three-dimensional image and spatial spectrum analysis of behavior of small animal erythrocytes in optical tweezers

Hui Chi Chen; Wen-Tai Shen; Yu-Han Kong; Chun-Hao Chuang

doi:10.1117/12.760571

29 February 2008 Three-dimensional image and spatial spectrum analysis of behavior of small animal erythrocytes in optical tweezers

Hui Chi Chen, Wen-Tai Shen, Yu-Han Kong, Chun-Hao Chuang

Author Affiliations +

Proceedings Volume 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI; 685912 (2008) https://doi.org/10.1117/12.760571
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Because of the softness of membrane, erythrocytes (red blood cell, RBC) have different shapes while being immersed in buffer with different osmotic pressure. While affecting by different viruses and illnesses, RBC may change its shape, or its membrane may become rigid. Moreover, RBC will ford and stretch when it is trapped by optical tweezers. Therefore, the behaviors of RBC in optical tweezers raise more discussion. In this report, we set up an optical tweezers to trap RBC of small animals like feline and canine. By adding a long working distance objective to collect the side-viewing image, a 3-D image system was constructed to detect the motion of trapped RBC. To improve the image quality for side-view, an aperture and narrow glass plate were used. From the video of these images and their spatial spectrum, the shape of trapped RBC was studied.

Citation Download Citation

Hui Chi Chen, Wen-Tai Shen, Yu-Han Kong, and Chun-Hao Chuang "Three-dimensional image and spatial spectrum analysis of behavior of small animal erythrocytes in optical tweezers", Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 685912 (29 February 2008); https://doi.org/10.1117/12.760571

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