A fluorescence lifetime imaging microscopy (FLIM) system for the characterization of haematoxylin and eosin stained sample

U. S. Dinish; C. Y. Fu; B. K. Ng; T. H. Chow; V. M. Murukeshan; L. K. Seah; S. K. Tan

doi:10.1117/12.764412

28 February 2008 A fluorescence lifetime imaging microscopy (FLIM) system for the characterization of haematoxylin and eosin stained sample

U. S. Dinish, C. Y. Fu, B. K. Ng, T. H. Chow, V. M. Murukeshan, L. K. Seah, S. K. Tan

Author Affiliations +

Proceedings Volume 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI; 68590C (2008) https://doi.org/10.1117/12.764412
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We present the implementation of a fluorescence lifetime imaging microscopy (FLIM) system for cellular characterisation. FLIM system can be used as an investigative tool to identify minor biochemical changes in cellular abnormalities. These subtle changes could possibly alter cellular fluorescence properties such as emission wavelength and lifetime. In this study, the fluorescence lifetime of haematoxylin and eosin (H&E)-stained tissues were investigated using a wide-field time-domain FLIM system. The transient response of epithelial fluorescence was investigated and the lifetime extracted using a bi-exponential model. It was found that the fluorescence lifetimes of eosin can be correlated to the tissue histology. The preliminary result suggests that tumor-associated molecules are retained in the tissues even after tissue fixation and staining. The developed FLIM system was successfully applied to detect the histological changes in the tissue samples. Optimization of system parameters is also discussed.

Citation Download Citation

U. S. Dinish, C. Y. Fu, B. K. Ng, T. H. Chow, V. M. Murukeshan, L. K. Seah, and S. K. Tan "A fluorescence lifetime imaging microscopy (FLIM) system for the characterization of haematoxylin and eosin stained sample", Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 68590C (28 February 2008); https://doi.org/10.1117/12.764412

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