Characterization of dermal structural assembly in normal and pathological connective tissues by intrinsic signal multiphoton optical microscopy

Julia G. Lyubovitsky; Xiaoman Xu; Chung-ho Sun; Bogi Andersen; Tatiana B. Krasieva; Bruce J. Tromberg

doi:10.1117/12.762702

7 February 2008 Characterization of dermal structural assembly in normal and pathological connective tissues by intrinsic signal multiphoton optical microscopy

Julia G. Lyubovitsky, Xiaoman Xu, Chung-ho Sun, Bogi Andersen, Tatiana B. Krasieva, Bruce J. Tromberg

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Proceedings Volume 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI; 685902 (2008) https://doi.org/10.1117/12.762702
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Employing a reflectance multi-photon microscopy (MPM) technique, we developed novel method to quantitatively study the three-dimensional assembly of structural proteins within bulk of dermal ECMs. Using a structurally simplified model of skin with enzymatically dissected epidermis, we find that low resolution MPM clearly discriminates between normal and pathological dermis. High-resolution images revealed that the backscattered MPM signals are affected by the assembly of collagen fibrils and fibers within this system. Exposure of tissues to high concentrations of potentially denaturing chemicals also resulted in the reduction of SHG signals from structural proteins which coincided with the appearance of aggregated fluorescent structures.

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Julia G. Lyubovitsky, Xiaoman Xu, Chung-ho Sun, Bogi Andersen, Tatiana B. Krasieva, and Bruce J. Tromberg "Characterization of dermal structural assembly in normal and pathological connective tissues by intrinsic signal multiphoton optical microscopy", Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 685902 (7 February 2008); https://doi.org/10.1117/12.762702

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