Smart bombing a single targeted cell with femtogram order reagents using laser-induced shockwave technique

Kazunori Okano; Noriko Takizawa; Takayuki Uwada; Yoichiroh Hosokawa; Hiroshi Masuhara

doi:10.1117/12.762613

25 February 2008 Smart bombing a single targeted cell with femtogram order reagents using laser-induced shockwave technique

Kazunori Okano, Noriko Takizawa, Takayuki Uwada, Yoichiroh Hosokawa, Hiroshi Masuhara

Author Affiliations +

Proceedings Volume 6854, Optical Interactions with Tissue and Cells XIX; 685414 (2008) https://doi.org/10.1117/12.762613
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Injection and delivery of small amount reagent in aqueous solution for cell chip was performed utilizing regeneratively amplified femtosecond laser system. In our new trial, the reagent integrated on a solid strip are released and delivered to targeted cells with the femutosecond laser-induced impulsive-force. The reagent was fixed in poly(vinyl alcohol) or polystyrene film on a glass-substrate strip. When a single pulsed femtosecond laser was focused in the solution, the film near the focal point was fragmented and the reagent was dispersed in 45-μm φ area at 50 μm from the surface of the reagent strip. As examples cardiomyocyte beating cells of P19CL6 were bombed with epinephrine and acetylcholine, and as a result the beating ratio of the cells were quickly stimulated and suppressed, respectively. The results demonstrate that the present method is a promising key nano/micro technology for diagnosis and drug discovery.

Citation Download Citation

Kazunori Okano, Noriko Takizawa, Takayuki Uwada, Yoichiroh Hosokawa, and Hiroshi Masuhara "Smart bombing a single targeted cell with femtogram order reagents using laser-induced shockwave technique", Proc. SPIE 6854, Optical Interactions with Tissue and Cells XIX, 685414 (25 February 2008); https://doi.org/10.1117/12.762613

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