UV-B radiation induced effects on human T-lymphocytes

Gianfranco Giubileo; Agostina Congiu Castellano; Silvia Gaudenzi; Paola Grimaldi; Deleana Pozzi; Adriana Puiu

doi:10.1117/12.757866

11 September 2007 UV-B radiation induced effects on human T-lymphocytes

Gianfranco Giubileo, Agostina Congiu Castellano, Silvia Gaudenzi, Paola Grimaldi, Deleana Pozzi, Adriana Puiu

Proceedings Volume 6785, ROMOPTO 2006: Eighth Conference on Optics; 67851H (2007) https://doi.org/10.1117/12.757866
Event: ROMOPTO 2006: Eighth Conference on Optics, 2006, Sibiu, Romania

Abstract

The reactive oxygen species (ROS) produced inside cells by UV-B radiation may induce apoptosis, a process that realizes the programmed death of cells. In the present paper the UV induced damage was studied in a human T-Lymphocyte cell line (the Jurkat line) by mean of IR laser photoacoustic spectroscopy (PAS) and by Fourier Transform Infrared Spectroscopy (FTIR) combined with biological assays based on flow cytometry. The apoptosis was induced in vitro in the Jurkat samples by exposition to UV-B radiation with a dose of 310 mJ/cm². PAS measurements were performed through a 10W c.w. CO₂ laser based optical system realised at ENEA Molecular Spectroscopy Laboratory in Frascati (Italy).

Citation Download Citation

Gianfranco Giubileo, Agostina Congiu Castellano, Silvia Gaudenzi, Paola Grimaldi, Deleana Pozzi, and Adriana Puiu "UV-B radiation induced effects on human T-lymphocytes", Proc. SPIE 6785, ROMOPTO 2006: Eighth Conference on Optics, 67851H (11 September 2007); https://doi.org/10.1117/12.757866

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