Analysis of cellular structure and dynamics with digital holographic microscopy

P. Marquet; B. Rappaz; F. Charrière; Y. Emery; C. Depeursinge; P. Magistretti

doi:10.1117/12.738579

13 July 2007 Analysis of cellular structure and dynamics with digital holographic microscopy

P. Marquet, B. Rappaz, F. Charrière, Y. Emery, C. Depeursinge, P. Magistretti

Proceedings Volume 6633, Biophotonics 2007: Optics in Life Science; 66330F (2007) https://doi.org/10.1117/12.738579
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically, the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We discuss some approaches allowing to directly obtain separate measurements of the thickness and the refractive index (RI) of a given living cell from the phase signal.

Citation Download Citation

P. Marquet, B. Rappaz, F. Charrière, Y. Emery, C. Depeursinge, and P. Magistretti "Analysis of cellular structure and dynamics with digital holographic microscopy", Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66330F (13 July 2007); https://doi.org/10.1117/12.738579

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