Monitoring cellular metabolic pathways by wavelength- and time-resolved intracellular autofluorescence

Yicong Wu; Wei Zheng; Jianan Y. Qu

doi:10.1117/12.727610

11 July 2007 Monitoring cellular metabolic pathways by wavelength- and time-resolved intracellular autofluorescence

Yicong Wu, Wei Zheng, Jianan Y. Qu

Proceedings Volume 6628, Diagnostic Optical Spectroscopy in Biomedicine IV; 662806 (2007) https://doi.org/10.1117/12.727610
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Autofluorescence spectroscopy has been a widely explored noninvasive technique to detect the precancerous development in epithelial tissue, where NADH and FAD fluorescence are metabolism related. In this study, we investigated the methods to monitor cellular metabolism based on the ratio of NADH over FAD fluorescence and the ratio of free NADH and protein-bound NADH fluorescence, respectively. The signals of free NADH, protein-bound NADH and FAD were isolated from the intracellular autofluorescence using wavelength- and time-resolved fluorescence spectroscopy. We demonstrated that the wavelength- and time-resolved intracellular autofluorescence can be used to monitor the cellular metabolic pathways and differentiate the normal cells from the cancer cells.

Citation Download Citation

Yicong Wu, Wei Zheng, and Jianan Y. Qu "Monitoring cellular metabolic pathways by wavelength- and time-resolved intracellular autofluorescence", Proc. SPIE 6628, Diagnostic Optical Spectroscopy in Biomedicine IV, 662806 (11 July 2007); https://doi.org/10.1117/12.727610

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