Three-dimensional Fourier-domain optical coherence tomography of alveolar mechanics in stepwise inflated and deflated isolated and perfused rabbit lungs

Alexander Krueger; Lilla Knels; Sven Meissner; Martina Wendel; Axel R. Heller; Thomas Lambeck; Thea Koch; Edmund Koch

doi:10.1117/12.727891

11 July 2007 Three-dimensional Fourier-domain optical coherence tomography of alveolar mechanics in stepwise inflated and deflated isolated and perfused rabbit lungs

Alexander Krueger, Lilla Knels, Sven Meissner, Martina Wendel, Axel R. Heller, Thomas Lambeck, Thea Koch, Edmund Koch

Author Affiliations +

Proceedings Volume 6627, Optical Coherence Tomography and Coherence Techniques III; 662707 (2007) https://doi.org/10.1117/12.727891
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Fourier domain optical coherence tomography (FD-OCT) was used to acquire three-dimensional image stacks of isolated and perfused rabbit lungs (n = 4) at different constant pulmonary airway pressures (CPAP) and during vascular fixation. After despeckling and applying a threshold, the images were segmented into air and tissue, and registered to each other to compensate for movement between CPAP steps. The air-filled cross-sectional areas were quantified using a semi-automatic algorithm. The cross-sectional area of alveolar structures taken at all three perpendicular planes increased with increasing CPAP. Between the minimal CPAP of 3 mbar and the maximum of 25 mbar the areas increased to about 140% of their initial value. There was no systematic dependency of inflation rate on initial size of the alveolar structure. During the perfusion fixation of the lungs with glutaraldehyde morphometric changes of the alveolar geometry measured with FD-OCT were negligible.

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Alexander Krueger, Lilla Knels, Sven Meissner, Martina Wendel, Axel R. Heller, Thomas Lambeck, Thea Koch, and Edmund Koch "Three-dimensional Fourier-domain optical coherence tomography of alveolar mechanics in stepwise inflated and deflated isolated and perfused rabbit lungs", Proc. SPIE 6627, Optical Coherence Tomography and Coherence Techniques III, 662707 (11 July 2007); https://doi.org/10.1117/12.727891

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