Fibre-optic sensors for the estimation of biofilm thickness on metals

A. Balaji Ganesh; T. K. Radhakrishnan; G. Gobi; D. Sastikumar

doi:10.1117/12.738423

2 July 2007 Fibre-optic sensors for the estimation of biofilm thickness on metals

A. Balaji Ganesh, T. K. Radhakrishnan, G. Gobi, D. Sastikumar

Proceedings Volume 6619, Third European Workshop on Optical Fibre Sensors; 66191E (2007) https://doi.org/10.1117/12.738423
Event: Third European Workshop on Optical Fibre Sensors, 2007, Napoli, Italy

Abstract

This paper presents an optical approach to estimate the thickness of biofilms on metals in natural aqueous environment. An in-situ fibre optic sensor is held above the sample surface, to offer real time information continuously by measuring the spatially resolved profiles of scattered light in biofilms. The optical results correlate well with the biofilm thickness which is measured using a microscope and a microcapillary with a protruding thin, long flexible wire from the top of the microcapillary. The biofilm thickness is less than 120 &mgr;m on test material after 20 days. Microscopic image analysis also provides the evidence to confirm micro colony formation by rod and cocci bacterial species. The species Klebsiella are found to be dominant on carbon steel. It is observed that measured optical intensity is proportional to the concentration of organisms.

Citation Download Citation

A. Balaji Ganesh, T. K. Radhakrishnan, G. Gobi, and D. Sastikumar "Fibre-optic sensors for the estimation of biofilm thickness on metals", Proc. SPIE 6619, Third European Workshop on Optical Fibre Sensors, 66191E (2 July 2007); https://doi.org/10.1117/12.738423

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