Evanescent optical fluorescence excitation: the role of photonic mode density

Bernhard Menges; Akihiro Sato; Wolfgang Knoll

doi:10.1117/12.721103

3 May 2007 Evanescent optical fluorescence excitation: the role of photonic mode density

Bernhard Menges, Akihiro Sato, Wolfgang Knoll

Proceedings Volume 6556, Micro (MEMS) and Nanotechnologies for Defense and Security; 65560G (2007) https://doi.org/10.1117/12.721103
Event: Defense and Security Symposium, 2007, Orlando, Florida, United States

Abstract

In this paper we will discuss a few basic concepts concerning the use of evanescent optical fields for the excitation of fluorescent chromophores placed near the interface. The observation of enhanced fluorescence from chromophores excited by surface plasmon and waveguide modes will be presented and discussed. We attribute the enhancement to the near-field interaction between the chromophores and the increased photonic mode density by surface plasmon and waveguide modes. We determined limits of detection (LOD) of DNA hybridisation using the same sensor architecture by surface plasmon fluorescence spectroscopy (SPFS) and optical waveguide fluorescence spectroscopy (OWFS). Both SPFS and OWFS techniques have the same detection principle using an enhanced electromagnetic field to excite fluorophores and make it possible to monitor DNA hybridisation in real-time with high sensitivity. The relative photonic mode density of each mode was calculated under the resonance condition, and these values are reflected in the LOD values.

Citation Download Citation

Bernhard Menges, Akihiro Sato, and Wolfgang Knoll "Evanescent optical fluorescence excitation: the role of photonic mode density", Proc. SPIE 6556, Micro (MEMS) and Nanotechnologies for Defense and Security, 65560G (3 May 2007); https://doi.org/10.1117/12.721103

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available