Changes in visible light transmission across the corneal stroma

James Doutch; Andrew J. Quantock; Keith M. Meek

doi:10.1117/12.740957

26 April 2007 Changes in visible light transmission across the corneal stroma

James Doutch, Andrew J. Quantock, Keith M. Meek

Proceedings Volume 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII; 65350Q (2007) https://doi.org/10.1117/12.740957
Event: Saratov Fall Meeting 2006, 2006, Saratov, Russian Federation

Abstract

The light scattering properties of the corneal stroma outside of the central region do not appear to have been studied in the literature. This is curious given variations in ultrastructure which are known to occur as a function of position in the central and peripheral cornea. Human and bovine corneas with limiting cell layers removed were equilibriated by dialysis close to physiological hydration. Spectral transmittance was then measured in the 400-700nm wavelength range. Readings were taken at 1mm intervals radiating out from the corneal centre, in one meridional plane. Results show transmittance gradually decreasing within the central 4mm portion of the cornea, which were modelled by the known tissue thickness increase. Outside this region an extra contributory term to extinction is observed; we briefly investigate possible ultrastructural contributors to this.

Citation Download Citation

James Doutch, Andrew J. Quantock, and Keith M. Meek "Changes in visible light transmission across the corneal stroma", Proc. SPIE 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII, 65350Q (26 April 2007); https://doi.org/10.1117/12.740957

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