Application of surface-enhanced Raman toward the detection of cell membrane proteins

Li-Lin Tay; Qingyan Hu; Matthew Noestheden; John P. Pezacki

doi:10.1117/12.700402

14 February 2007 Application of surface-enhanced Raman toward the detection of cell membrane proteins

Li-Lin Tay, Qingyan Hu, Matthew Noestheden, John P. Pezacki

Proceedings Volume 6450, Plasmonics in Biology and Medicine IV; 645003 (2007) https://doi.org/10.1117/12.700402
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Silver nanoparticles (Ag-NPs) functionalized with molecule probes which contains unique Raman vibrational mode as well as a recognition binding site to target specific surface proteins expressed on the transfected human cervical cancel cells was utilized to detect the cell surface protein through surface enhanced Raman scattering (SERS) microscopy. In this study, we demonstrate that only the aggregated Ag-NPs displays detectable SERS signal. We also observed striking polarization anisotropy in many dimer or trimer NP aggregates. This work will impact on the future design of NP clusters for in-vivo cell imaging.

Citation Download Citation

Li-Lin Tay, Qingyan Hu, Matthew Noestheden, and John P. Pezacki "Application of surface-enhanced Raman toward the detection of cell membrane proteins", Proc. SPIE 6450, Plasmonics in Biology and Medicine IV, 645003 (14 February 2007); https://doi.org/10.1117/12.700402

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