Paper
5 February 2007 Nanosecond imaging of quantum dots in highly fluorescent media
Andrew C. Mitchell, Christopher G. Morgan
Author Affiliations +
Abstract
Quantum dots are now recognised as valuable luminescent labels. Amongst their desirable characteristics is a relatively long luminescence decay time that can allow selective detection in the presence of shorter lived background fluorescence. Here we demonstrate that nanosecond time-resolved imaging of quantum dot samples is easily accomplished with a directly-gated cooled CCD system that does not require an image intensifier stage to achieve nanosecond time resolution. The approach is likely to find application both to imaging of quantum dots in the presence of autofluorescence from tissues and also for time-resolved measurements of resonance energy transfer from quantum dot donors to conventional acceptor labels.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Andrew C. Mitchell and Christopher G. Morgan "Nanosecond imaging of quantum dots in highly fluorescent media", Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 64480Q (5 February 2007); https://doi.org/10.1117/12.710613
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CITATIONS
Cited by 3 scholarly publications.
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KEYWORDS
Quantum dots

Luminescence

Charge-coupled devices

Fluorescence resonance energy transfer

Imaging systems

Cameras

Tissues

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