Quantum dot-DNA bioconjugates for fluorescence-resonance-energy-transfer-based biosensing

Igor L. Medintz; Lorenzo Berti; Thomas Pons; Hedi Mattoussi

doi:10.1117/12.699384

27 February 2007 Quantum dot-DNA bioconjugates for fluorescence-resonance-energy-transfer-based biosensing

Igor L. Medintz, Lorenzo Berti, Thomas Pons, Hedi Mattoussi

Proceedings Volume 6448, Colloidal Quantum Dots for Biomedical Applications II; 64480G (2007) https://doi.org/10.1117/12.699384
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Semiconductor quantum dots (QDs) have unique photophysical properties which make them excellent fluorescence resonance energy transfer donors. However, lack of facile methods for conjugating biomolecules such as DNA, proteins and peptides to QDs have limited their applications. In this report, we describe a general procedure for the preparation of a synthetic peptide that can be covalently attached to DNA segments and used to facilitate the self-assembly of the modified DNA onto water soluble QDs. To characterize this conjugation strategy, dye-labeled DNA is first reacted with the synthetic peptide and the resulting peptide-DNA then self-assembled onto QDs. QD attachment is verified by monitoring resonance energy transfer efficiency from the QD donor to the dye-labeled DNA acceptor. QD-DNA bioconjugates assembled using this method may find applications as molecular beacons and hybridization probes.

Citation Download Citation

Igor L. Medintz, Lorenzo Berti, Thomas Pons, and Hedi Mattoussi "Quantum dot-DNA bioconjugates for fluorescence-resonance-energy-transfer-based biosensing", Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 64480G (27 February 2007); https://doi.org/10.1117/12.699384

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