Optical imaging the redox status change during cell apoptosis

Ting Su; Zhihong Zhang; Juqiang Lin; Qingming Luo

doi:10.1117/12.701654

13 February 2007 Optical imaging the redox status change during cell apoptosis

Ting Su, Zhihong Zhang, Juqiang Lin, Qingming Luo

Proceedings Volume 6438, Biophotonics and Immune Responses II; 64380K (2007) https://doi.org/10.1117/12.701654
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Many cellular events involve the alteration in redox equilibrium, globally or locally. In many cases, excessive reactive oxygen species (ROS) production is the underlying cause. Several green fluoresecence protein based indicators are constructed to measure redox status in cells, e.g, rxYFP and roGFPs, which allow real time detection. reduction and oxidization-sensitive GFP (RoGFPs) are more useful due to ratiometric variation by excitation, making the measurement more accurate. Utilizing one of those roGFPs called roGFP1, we establish a mitochondrial redox state probing platform in HeLa cells with laser scan confocal microscopy (LSCM) as detection system. Control experiments confirmed that our platform could produce stable ratiometric values, which made the data more accurately reflect the real environmental changes of redox status that roGFP1 probed. Using exogenous H₂O₂ and DTT, we evaluated the reactivity and reversibility of roGFP1. The minimal hydrogen peroxide concentration that roGFP1 could show detectable ratiometric changes in our system was about 200&mgr;M. Preliminarily applying our platform to exploring the redox status during apoptosis, we observed an increase in ratiometric, suggesting an excessive ROS production.

Citation Download Citation

Ting Su, Zhihong Zhang, Juqiang Lin, and Qingming Luo "Optical imaging the redox status change during cell apoptosis", Proc. SPIE 6438, Biophotonics and Immune Responses II, 64380K (13 February 2007); https://doi.org/10.1117/12.701654

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Cell death

Luminescence

Oxygen

Confocal microscopy

Green fluorescent protein

Imaging systems

Proteins

Show All Keywords

Keywords/Phrases

Search In:

Publication Years