Fluorescence tomography in a murine model of Alzheimer’s disease

Scott B. Raymond; Anand T. N. Kumar; Andrew K. Dunn; David A. Boas; Brian J. Bacskai

doi:10.1117/12.700199

13 February 2007 Fluorescence tomography in a murine model of Alzheimer's disease

Scott B. Raymond, Anand T. N. Kumar, Andrew K. Dunn, David A. Boas, Brian J. Bacskai

Proceedings Volume 6434, Optical Tomography and Spectroscopy of Tissue VII; 64342H (2007) https://doi.org/10.1117/12.700199
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Noninvasive molecular imaging of amyloid plaques in murine Alzheimer's disease models would accelerate drug development and basic Alzheimer's research. Amyloid plaques differ from traditional fluorescent targets in size and spatial distribution and therefore present a unique challenge for biomarker development and tomography. To study imaging feasibility and establish biomarker criteria, we developed a digital mouse head model from a 100 &mgr;m-resolution, digital, segmented mouse atlas¹. The cortical region of the brain was filled with a spatially uniform distribution of plaques that had different fluorescent properties from the surrounding brain tissue, similar to current transgenic mouse models of Alzheimer's disease. Fluorescence was simulated with a Monte Carlo algorithm using different plaque densities, detection geometries, and background fluorescence. Our preliminary results demonstrated that shielding effects might require nonlinear reconstruction algorithms and that background fluorescence would seriously hinder quantitative burden estimation. The Monte Carlo based approach presented here offers a powerful way to study the feasibility of non-invasive imaging in murine Alzheimer's models and to optimize experimental conditions.

Citation Download Citation

Scott B. Raymond, Anand T. N. Kumar, Andrew K. Dunn, David A. Boas, and Brian J. Bacskai "Fluorescence tomography in a murine model of Alzheimer's disease", Proc. SPIE 6434, Optical Tomography and Spectroscopy of Tissue VII, 64342H (13 February 2007); https://doi.org/10.1117/12.700199

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available