A direct approach to time domain fluorescence tomography based on asymptotic lifetime analysis

Anand T. N. Kumar; Scott B. Raymond; David A. Boas; Brian J. Bacskai

doi:10.1117/12.699692

1 March 2007 A direct approach to time domain fluorescence tomography based on asymptotic lifetime analysis

Anand T. N. Kumar, Scott B. Raymond, David A. Boas, Brian J. Bacskai

Proceedings Volume 6434, Optical Tomography and Spectroscopy of Tissue VII; 64340E (2007) https://doi.org/10.1117/12.699692
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

A theoretical framework is presented that allows a lifetime based analysis of the entire temporal diffuse fluorescence response curve from a turbid medium. Optimization studies using singular value decomposition analysis show that direct time domain fluorescence reconstructions are optimally performed using a few points near the peak and rise portions of the temporal response. It is also shown that the initial portion of the fluorescent response curve offers superior contrast-to-noise performance, while the late decay portions offer minimal cross-talk between multiple lifetime components.

Citation Download Citation

Anand T. N. Kumar, Scott B. Raymond, David A. Boas, and Brian J. Bacskai "A direct approach to time domain fluorescence tomography based on asymptotic lifetime analysis", Proc. SPIE 6434, Optical Tomography and Spectroscopy of Tissue VII, 64340E (1 March 2007); https://doi.org/10.1117/12.699692

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