Evaluation of aptamers as molecular recognition elements for pathogens using capillary electrophoretic analysis

Sun McMasters; Dimitra N. Stratis-Cullum

doi:10.1117/12.686357

23 October 2006 Evaluation of aptamers as molecular recognition elements for pathogens using capillary electrophoretic analysis

Sun McMasters, Dimitra N. Stratis-Cullum

Author Affiliations +

Proceedings Volume 6380, Smart Medical and Biomedical Sensor Technology IV; 63800B (2006) https://doi.org/10.1117/12.686357
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

The biomolecular interactions between a fluorescently labeled aptamer and whole cell Campylobacter jejuni(C. jejuni) have been characterized using capillary electrophoresis with laser-induced fluorescence detection. From electrophoretic analysis, the bound complex forms, unbound aptamer, and cells were visualized. The relative binding affinity of the DNA aptamer with C. jejuni was compared with other food-borne pathogens including Escherichia coli O157:H7 and Salmonella typhimurium. Preliminary data suggests that this aptamer exhibits strong binding affinity towards C. jejuni with minimal cross reactivity over other food pathogens when equivalent cell concentrations were used.

Citation Download Citation

Sun McMasters and Dimitra N. Stratis-Cullum "Evaluation of aptamers as molecular recognition elements for pathogens using capillary electrophoretic analysis", Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 63800B (23 October 2006); https://doi.org/10.1117/12.686357

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