Patterned arrays for the efficient detection of whole cells

Troy A. Alexander

doi:10.1117/12.686360

23 October 2006 Patterned arrays for the efficient detection of whole cells

Troy A. Alexander

Proceedings Volume 6380, Smart Medical and Biomedical Sensor Technology IV; 638003 (2006) https://doi.org/10.1117/12.686360
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

Surface-Enhanced-Raman-Spectroscopy (SERS) is potentially a very sensitive technique for the detection of biological agents (i.e., proteins, viruses or whole cell bacteria). However, since initial reports, its utility has not been realized. Its limited acceptance as a routine analysis technique for both chemical and biological agents is largely due to the lack of reproducible SERS-active substrates. Most established SERS substrate fabrication schemes are based on selfassembly of the metallic (typically, Au, Ag, Pt, Pd or Cu) surfaces responsible for enhancement. Further, these protocols do not lend themselves to the stringent control over the enhancing feature shape, size, and placement on a nanometer scale. SERS can be made a more robust and attractive spectroscopic technique for biological agents by developing quantifiable, highly sensitive, and highly selective SERS-active substrates. Recently, novel SERS-active substrates, fabricated from nano-patterned Si and Au have been commercialized and are easily obtained in the marketplace. Commercialized Au SERS-active substrates fabricated using semiconductor manipulation and routine metal vapor deposition techniques used for the spectral analysis of intact bacterial cells. This talk will focus on the substrate characterization (microscopic and spectral) and application towards whole cells.

Citation Download Citation

Troy A. Alexander "Patterned arrays for the efficient detection of whole cells", Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 638003 (23 October 2006); https://doi.org/10.1117/12.686360

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