Optically controlled manipulation of live cells using optoelectronic tweezers

Aaron T. Ohta; Pei-Yu Chiou; Ming C. Wu

doi:10.1117/12.679710

5 October 2006 Optically controlled manipulation of live cells using optoelectronic tweezers

Aaron T. Ohta, Pei-Yu Chiou, Ming C. Wu

Proceedings Volume 6326, Optical Trapping and Optical Micromanipulation III; 632617 (2006) https://doi.org/10.1117/12.679710
Event: SPIE Optics + Photonics, 2006, San Diego, California, United States

Abstract

Optoelectronic tweezers (OET) provides a non-invasive, low-power, optical manipulation tool for trapping, transporting, and separating microparticles, cells, and other bioparticles. The OET device uses a photosensitive layer to form "virtual electrodes" upon exposure to light, creating non-uniformities in an applied electric field. The electric field gives rise to a force known as dielectrophoresis: microparticles move as a result of the non-uniformities in the electric field imparting unequal forces on the induced dipoles of the particles. These virtual electrodes can be actuated with low optical intensities, enabling the use of incoherent light sources and direct imaging techniques to create optical manipulation patterns in real-time. In this paper, we demonstrate OET operation on live cells, including the trapping and manipulation of red and white blood cells, and the automated collection of HeLa cells. Automated size-based sorting is performed on a mixture of 15- and 20-μm-diameter polystyrene beads, and dielectric property-based separation is used to differentiate between live and dead white blood cells.

Citation Download Citation

Aaron T. Ohta, Pei-Yu Chiou, and Ming C. Wu "Optically controlled manipulation of live cells using optoelectronic tweezers", Proc. SPIE 6326, Optical Trapping and Optical Micromanipulation III, 632617 (5 October 2006); https://doi.org/10.1117/12.679710

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