Optical trapping dynamics for cell identification

Giovanni Volpe; Gajendra P. Singh; Florian Buettner; Anna M. Drets; Dmitri V. Petrov

doi:10.1117/12.679374

30 August 2006 Optical trapping dynamics for cell identification

Giovanni Volpe, Gajendra P. Singh, Florian Buettner, Anna M. Drets, Dmitri V. Petrov

Proceedings Volume 6326, Optical Trapping and Optical Micromanipulation III; 63260P (2006) https://doi.org/10.1117/12.679374
Event: SPIE Optics + Photonics, 2006, San Diego, California, United States

Abstract

Living cells show a variety of morphological traits upon which numerous identification techniques have already been developed. However most of them involve lengthy biochemical procedures and can compromise the viability of the cell. We demonstrate a method to differentiate cells only on the basis of its trapping dynamics while it is being drawn into an optical trap (Optical Trapping Dynamics). Since it relies only on the inherent properties of the optical trap, without requiring external markers or biochemically sensitive spectroscopic techniques, it can be readily combined with existing optical tweezers setups. We applied it to the study of the yeast cell-cycle stages, showing, in particular, how it can be amenable for the measurement of the budding index of a cell population.

Citation Download Citation

Giovanni Volpe, Gajendra P. Singh, Florian Buettner, Anna M. Drets, and Dmitri V. Petrov "Optical trapping dynamics for cell identification", Proc. SPIE 6326, Optical Trapping and Optical Micromanipulation III, 63260P (30 August 2006); https://doi.org/10.1117/12.679374

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