Biological crystal alignment using image processing

Kazimierz J. Gofron; Krzysztof Lazarski; Michael Molitsky; Andrzej Joachimiak

doi:10.1117/12.706125

30 August 2006 Biological crystal alignment using image processing

Kazimierz J. Gofron, Krzysztof Lazarski, Michael Molitsky, Andrzej Joachimiak

Proceedings Volume 6312, Applications of Digital Image Processing XXIX; 63121W (2006) https://doi.org/10.1117/12.706125
Event: SPIE Optics + Photonics, 2006, San Diego, California, United States

Abstract

Crystal location and alignment to the x-ray beam is an enabling technology necessary for automation of the macromolecular crystallography at synchrotron beamlines. In a process of crystal structure determination, a small size x-ray synchrotron beam with FWHM as small as 70 μm (bending magnet beamlines) and 20 μm (undulator beamlines) is focused at or downstream of the crystal sample. Protein crystals used in structure determination become smaller and approach 50 μm or less, and need to be precisely placed in the focused x-ray beam. At the Structural Biology Center the crystals are mounted on a goniostat, allowing precise crystal xyz positioning and rotations. One low and two high magnification cameras integrated into synchrotron beamline permit imaging of the crystal mounted on a goniostat. The crystals are held near liquid nitrogen temperatures using cryostream to control secondary radiation damage. Image processing techniques are used for automatic and precise placing of protein crystals in synchrotron beam. Here we are discussing automatic crystal centering process considered for Structure Biology Center utilizing several image processing techniques.

Citation Download Citation

Kazimierz J. Gofron, Krzysztof Lazarski, Michael Molitsky, and Andrzej Joachimiak "Biological crystal alignment using image processing", Proc. SPIE 6312, Applications of Digital Image Processing XXIX, 63121W (30 August 2006); https://doi.org/10.1117/12.706125

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