Combinatory scanning confocal laser and acoustic vector contrast microscopy: multi-contrast imaging of soft matter samples

Albert E. Kamanyi Jr.; Reinhold Wannemacher; Wolfgang Grill

doi:10.1117/12.657911

15 March 2006 Combinatory scanning confocal laser and acoustic vector contrast microscopy: multi-contrast imaging of soft matter samples

Albert E. Kamanyi Jr., Reinhold Wannemacher, Wolfgang Grill

Author Affiliations +

Proceedings Volume 6177, Health Monitoring and Smart Nondestructive Evaluation of Structural and Biological Systems V; 617712 (2006) https://doi.org/10.1117/12.657911
Event: Nondestructive Evaluation for Health Monitoring and Diagnostics, 2006, San Diego, CA, United States

Abstract

Synchronous operation of a confocal laser scanning microscope (CLSM) and a confocal vector contrast scanning acoustic microscope (phase sensitive scanning acoustic microscopy: PSAM) has been developed. Imaging is performed on objects mounted on a cover slide with the CLSM operated in reflection through the slide with an immersion fluid and PSAM operating in water respectively aqueous solutions from the other side (half space). Examples involving living cells and soft matter samples illustrating various combinatory schemes and advantages of multi-contrast optical and acoustic contrast are demonstrated. This includes combinations with fluorescence microscopy and ultrasonic topographical imaging as well as combinatory three-dimensional imaging.

Citation Download Citation

Albert E. Kamanyi Jr., Reinhold Wannemacher, and Wolfgang Grill "Combinatory scanning confocal laser and acoustic vector contrast microscopy: multi-contrast imaging of soft matter samples", Proc. SPIE 6177, Health Monitoring and Smart Nondestructive Evaluation of Structural and Biological Systems V, 617712 (15 March 2006); https://doi.org/10.1117/12.657911

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