Ceramide accumulation after photosensitization is absent after the Bcl-2 Inhibitor HA14-1

Duska Separovic; Shouye Wang; Kentaro Hanada; David Kessel

doi:10.1117/12.657692

6 March 2006 Ceramide accumulation after photosensitization is absent after the Bcl-2 inhibitor HA14-1

Duska Separovic, Shouye Wang, Kentaro Hanada, David Kessel

Proceedings Volume 6139, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV; 61390E (2006) https://doi.org/10.1117/12.657692
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

The oxidative stress initiated by photodynamic therapy (PDT) results in accumulation of ceramide in various cell types undergoing apoptosis. Apoptotic responses to PDT are similar to those of the oxidative stress inducer and the Bcl-2 inhibitor HA 14-1 in murine leukemia L1210 cells. Here we tested whether de novo ceramide accumulation can be induced by HA 14-1 during apoptosis in Jurkat T human leukemia cells. Similar to photosensitization with Pc 4, treatment of Jurkat cells with HA14-1 leads to DEVDase activation and nuclear apoptosis. However, unlike Pc 4-PDT, HA 14-1 did not induce accumulation of de novo ceramide. The data suggest that, compared to HA 14-1, de novo ceramide accumulation is a distinct response to PDT in Jurkat cells.

Citation Download Citation

Duska Separovic, Shouye Wang, Kentaro Hanada, and David Kessel "Ceramide accumulation after photosensitization is absent after the Bcl-2 inhibitor HA14-1", Proc. SPIE 6139, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV, 61390E (6 March 2006); https://doi.org/10.1117/12.657692

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