Apoptotic inducing ability of a novel photosensitizing agent, Ge Sulfophthalocyanine on oesophageal and breast cancer cell lines

H. Abrahamse; T. Kresfelder; T. Horne; M. Cronje; T. Nyokong

doi:10.1117/12.641604

6 March 2006 Apoptotic inducing ability of a novel photosensitizing agent, Ge sulfophthalocyanine on oesophageal and breast cancer cell lines

H. Abrahamse, T. Kresfelder, T. Horne, M. Cronje, T. Nyokong

Author Affiliations +

Proceedings Volume 6139, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV; 613904 (2006) https://doi.org/10.1117/12.641604
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Photodynamic therapy (PDT) involves the use of a photosensitizer, which, when activated by light becomes toxic to the cancer cells. Lasers provide light at a specific wavelength required to activate the photosensitizer while the monochromaticity of the lasers at specific wavelengths results in maximum effectiveness of the photosensitizer during treatment. An important property of photosensitizers is that they should absorb light at a long wavelength as the light has to be able to penetrate tissue, and low energy light is able to travel further through tissue than light which absorbs at a shorter wavelength. This study aimed at evaluating the effects of 2 different photosensitizers, Al (AlPcSmix), commercially known as Photosens^(R) and Ge (GePcSmix), both from the Phthalocyanine family of sensitizers, on oesophageal (SNO) and breast cancer (MCF-7) cells. Cells were irradiated at 660nm with a power output of 100 mW and a fluence of 10 J/cm². Cell viability and proliferation were assessed using adenosine triphosphate (ATP) luminescence and alamarBlue^TM staining. Lactate dehydrogenase (LDH) activity was used as a measure of cytotoxicity while the Comet assay was used to evaluate DNA damage. Heat shock protein 70 (Hsp70) induction acted as a measure of cellular stress. Both photosensitizers used during the course of this study are effective in targeting malignant cells, and have a cytotoxic effect on these cells when activated using laser irradiation. However, cytotoxic effects were also measured in the absence of laser irradiation, indicating the importance of photosensitizer concentration. Lower concentrations of photosensitizer in the presence of laser irradiation showed greater apoptotic inducing ability than with high concentrations. Morphologically, cells were affected to the detriment despite viability tests indicating the contrary.

Citation Download Citation

H. Abrahamse, T. Kresfelder, T. Horne, M. Cronje, and T. Nyokong "Apoptotic inducing ability of a novel photosensitizing agent, Ge sulfophthalocyanine on oesophageal and breast cancer cell lines", Proc. SPIE 6139, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV, 613904 (6 March 2006); https://doi.org/10.1117/12.641604

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available