Chlorin <i>p<sub>6</sub></i> as a fluorescent probe for the investigation of surfactant-cyclodextrin interactions

Padmaja P Mishra; Ramkrishna Adhikary; Priyanka Lahiri; Anindya Datta

doi:10.1117/12.645958

10 February 2006 Chlorin p₆ as a fluorescent probe for the investigation of surfactant-cyclodextrin interactions

Padmaja P Mishra, Ramkrishna Adhikary, Priyanka Lahiri, Anindya Datta

Proceedings Volume 6097, Optical Molecular Probes for Biomedical Applications; 609708 (2006) https://doi.org/10.1117/12.645958
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Cyclodextrins (CD) are often projected as potential vehicles in targeted drug delivery. However, if the membrane structure is disrupted by CD, then it cannot be considered to be a good drug delivery vehicle. When an extrinsic fluorescence probe is used to monitor such interactions, there are no less than three possible equilibria that can operate simultaneously: surfactant-cyclodextrin, surfactant-fluorophore and cyclodextrin-fluorophore. The fluorescence intensity / lifetime might be affected by all these and so, the results depend strongly on the fluorophore used as well as the nature of the surfactant. This aspect highlights the importance of the suitability of the fluorescence probe to be used to study complicated systems and interaction. In the present work, chlorin p₆, prepared from chlorophyll from spinach leaves, has been used as the fluorescence probe to investigate the interaction between α-CD and β-CD with the neutral surfactants TX 100 and CTAB. The fluorophore is found to be a sensitive one for the study of the interaction of α- and β-CD with the surfactants Tritron X 100 (TX 100) and cetyl trimethyl ammonium bromide (CTAB). It is found that contrary to earlier reports, a complex between α-CD and TX 100 is formed, even though the binding constant is not very high. This observation can be obtained with chlorin p₆, which does not bind to the CDs, but not with a fluorophore like TNS, which binds to the CD as well and thus complicates the situation as the binding with CD is stronger than that between TX 100 and α-CD as compared to that between TNS and CD.

Citation Download Citation

Padmaja P Mishra, Ramkrishna Adhikary, Priyanka Lahiri, and Anindya Datta "Chlorin p₆ as a fluorescent probe for the investigation of surfactant-cyclodextrin interactions", Proc. SPIE 6097, Optical Molecular Probes for Biomedical Applications, 609708 (10 February 2006); https://doi.org/10.1117/12.645958

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