Locked nucleic acid molecular beacons for intracellular mRNA monitoring

Lin Wang; Chaoyong James Yang; Colin D. Medley; Steven A. Benner; Weihong Tan

doi:10.1117/12.644649

28 February 2006 Locked nucleic acid molecular beacons for intracellular mRNA monitoring

Lin Wang, Chaoyong James Yang, Colin D. Medley, Steven A. Benner, Weihong Tan

Proceedings Volume 6095, Nanobiophotonics and Biomedical Applications III; 60950M (2006) https://doi.org/10.1117/12.644649
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We have designed, synthesized, and investigated a novel molecular beacon (MB) using locked nucleic acid (LNA) bases for intracellular mRNA monitoring. This new LNA-MB has several useful properties including: very high melting temperature; excellent affinity for complementary sequences; superior single base mismatch discrimination capability; stablity against nuclease digestion; and not binding with single-stranded DNA binding proteins. All of these properties are highly advantageous for a molecular tool for various intracellular studies of biochemical, biological and medical significance.

Citation Download Citation

Lin Wang, Chaoyong James Yang, Colin D. Medley, Steven A. Benner, and Weihong Tan "Locked nucleic acid molecular beacons for intracellular mRNA monitoring", Proc. SPIE 6095, Nanobiophotonics and Biomedical Applications III, 60950M (28 February 2006); https://doi.org/10.1117/12.644649

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