Synthesis and characterization of peptide nanostructures designed for sensing applications

Normand Voyer; Mathieu Arseneault; Francois Otis

doi:10.1117/12.628791

12 October 2005 Synthesis and characterization of peptide nanostructures designed for sensing applications

Normand Voyer, Mathieu Arseneault, Francois Otis

Proceedings Volume 5969, Photonic Applications in Biosensing and Imaging; 59690M (2005) https://doi.org/10.1117/12.628791
Event: Photonics North, 2005, Toronto, Canada

Abstract

We report the design and the synthesis of membrane-active peptide nanostructures, as well as their use as signal transducer in a fluorimetric assay for biologically relevant analytes. Addition of hydrophobic 21-residue peptides bearing six crown ether side chains to a solution of small unilamellar vesicles loaded with a pH-sensitive fluorophore induces a rapid fluorescence increase associated with Na⁺/H⁺ transport across the bilayer membrane. To demonstrate the usefulness of these peptide nanostructures in the development of simple, rapid, and sensitive detection assays for a wide range of analytes, peptide nanostructures bearing a biotin at the N-terminal position were prepared. Addition of avidin to the assay employing these modified peptides resulted in a significant change in the time-dependent fluorescence profile. Control experiments with a non-binding proteins and saturated avidin showed that the observed changes are indeed due to specific binding of avidin to biotin modified nanostructures.

Citation Download Citation

Normand Voyer, Mathieu Arseneault, and Francois Otis "Synthesis and characterization of peptide nanostructures designed for sensing applications", Proc. SPIE 5969, Photonic Applications in Biosensing and Imaging, 59690M (12 October 2005); https://doi.org/10.1117/12.628791

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