Optical fibre biosensors for oxygen and glucose monitoring

J. S. Young; Patricia J. Scully; F. Kvasnik; K. Rose; Gabriela Kuncova; O. Podrazky; Vlastimil Matejec; Jan Mrazek

doi:10.1117/12.623688

23 May 2005 Optical fibre biosensors for oxygen and glucose monitoring

J. S. Young, Patricia J. Scully, F. Kvasnik, K. Rose, Gabriela Kuncova, O. Podrazky, Vlastimil Matejec, Jan Mrazek

Proceedings Volume 5855, 17th International Conference on Optical Fibre Sensors; (2005) https://doi.org/10.1117/12.623688
Event: 17th International Conference on Optical Fibre Sensors, 2005, Bruges, Belgium

Abstract

An optical fibre biosensor that uses an oxygen sensitive coating; Ruthenium complex [Dichlorotris(1,10-phenantroline)-ruthenium(II) hydrate], incorporated into an adhesive inorganic-organic hybrid polymer coating (ORMOCER) is described. The Ruthenium/ ORMOCER layer is used with optical fibres to form an extrinsic or intrinsic sensor. It can be applied to a microscope slide or lens to be interrogated by optical fibres, or form a cladding layer for an evanescent field optical fibre sensor. The Ruthenium complex is caused to fluoresce by a high brightness blue LED at 470 nm and the excitation light at 600nm is detected by a photomultiplier tube used as a photon counter, to measure fluorescence lifetime. The fluorescence is quenched by oxygen depletion within the layer, which can be linked to glucose by incorporation of suitable enzymes. The detection threshold is 0.7mg(O2)/litre, and the sensitivity is 70 ns/mg per litre.

Citation Download Citation

J. S. Young, Patricia J. Scully, F. Kvasnik, K. Rose, Gabriela Kuncova, O. Podrazky, Vlastimil Matejec, and Jan Mrazek "Optical fibre biosensors for oxygen and glucose monitoring", Proc. SPIE 5855, 17th International Conference on Optical Fibre Sensors, (23 May 2005); https://doi.org/10.1117/12.623688

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