Femtosecond laser-induced calcium release in neural-type cells

Nicholas Isaac Smith; Shigeki Iwanaga; Taro Beppu; Katsumasa Fujita; Osamu Nakamura; Satoshi Kawata

doi:10.1117/12.596923

28 April 2005 Femtosecond laser-induced calcium release in neural-type cells

Nicholas Isaac Smith, Shigeki Iwanaga, Taro Beppu, Katsumasa Fujita, Osamu Nakamura, Satoshi Kawata

Proceedings Volume 5705, Nanobiophotonics and Biomedical Applications II; (2005) https://doi.org/10.1117/12.596923
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Here we show that femtosecond laser irradiation can be used to evoke dynamic calcium concentration changes in living cells. The relatively localized interaction that results from the two-photon absorption process allows the release of calcium from intracellular stores in cells in vitro. The self-catalytic response to calcium elevation in a cell can increase the initial release of calcium further so that the entire cell undergoes a rise in cytosolic calcium concentration (i.e. a calcium wave). The calcium stimulation was observed in HeLa (non-excitable) and PC12 (excitable) cells, and could be seen to occur inside a range of power levels between approximately 20 to 80mW. The observation of direct calcium release by femtosecond laser which leads to a calcium wave in the cell has implications for photolytic calcium uncaging experiments since it could be a competing, or even dominant factor in some experiments using caged calcium for the generation of calcium waves.

Citation Download Citation

Nicholas Isaac Smith, Shigeki Iwanaga, Taro Beppu, Katsumasa Fujita, Osamu Nakamura, and Satoshi Kawata "Femtosecond laser-induced calcium release in neural-type cells", Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); https://doi.org/10.1117/12.596923

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