SERS quantitative urine creatinine measurement of human subject

Tsuei Lian Wang; Hui-hua Kenny Chiang; Hui-hsin Lu; Yung-da Hung

doi:10.1117/12.591393

31 March 2005 SERS quantitative urine creatinine measurement of human subject

Tsuei Lian Wang, Hui-hua Kenny Chiang, Hui-hsin Lu, Yung-da Hung

Proceedings Volume 5703, Plasmonics in Biology and Medicine II; (2005) https://doi.org/10.1117/12.591393
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

SERS method for biomolecular analysis has several potentials and advantages over traditional biochemical approaches, including less specimen contact, non-destructive to specimen, and multiple components analysis. Urine is an easily available body fluid for monitoring the metabolites and renal function of human body. We developed surface-enhanced Raman scattering (SERS) technique using 50nm size gold colloidal particles for quantitative human urine creatinine measurements. This paper shows that SERS shifts of creatinine (104mg/dl) in artificial urine is from 1400cm^-1 to 1500cm^-1 which was analyzed for quantitative creatinine measurement. Ten human urine samples were obtained from ten healthy persons and analyzed by the SERS technique. Partial least square cross-validation (PLSCV) method was utilized to obtain the estimated creatinine concentration in clinically relevant (55.9mg/dl to 208mg/dl) concentration range. The root-mean square error of cross validation (RMSECV) is 26.1mg/dl. This research demonstrates the feasibility of using SERS for human subject urine creatinine detection, and establishes the SERS platform technique for bodily fluids measurement.

Citation Download Citation

Tsuei Lian Wang, Hui-hua Kenny Chiang, Hui-hsin Lu, and Yung-da Hung "SERS quantitative urine creatinine measurement of human subject", Proc. SPIE 5703, Plasmonics in Biology and Medicine II, (31 March 2005); https://doi.org/10.1117/12.591393

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